Turroni S, Vitali B, Bendazzoli C, Candela M, Gotti R, Federici F, Pirovano F, Brigidi P
Department of Pharmaceutical Sciences, CIRB-Center for Biotechnology, University of Bologna, Bologna, Italy.
J Appl Microbiol. 2007 Nov;103(5):1600-9. doi: 10.1111/j.1365-2672.2007.03388.x.
This study was undertaken to evaluate the oxalate-degrading activity in several Lactobacillus species widely used in probiotic dairy and pharmaceutical preparations. Functional characterization of oxalyl-CoA decarboxylase and formyl-CoA transferase in Lactobacillus acidophilus was performed in order to assess the possible contribution of Lactobacillus in regulating the intestinal oxalate homeostasis.
In order to determine the oxalate-degrading ability in 60 Lactobacillus strains belonging to 12 species, a screening was carried out by using an enzymatic assay. A high variability in the oxalate-degrading capacity was found in the different species. Strains of Lact. acidophilus and Lactobacillus gasseri showed the highest oxalate-degrading activity. Oxalyl-CoA decarboxylase and formyl-CoA transferase genes from Lact. acidophilus LA14 were cloned and sequenced. The activity of the recombinant enzymes was assessed by capillary electrophoresis.
Strains of Lactobacillus with a high oxalate-degrading activity were identified. The function and significance of Lact. acidophilus LA14 oxalyl-CoA decarboxylase and formyl-CoA transferase in oxalate catabolism were demonstrated. These results suggest the potential use of Lactobacillus strains for the degradation of oxalate in the human gut.
Identification of probiotic strains with oxalate-degrading activity can offer the opportunity to provide this capacity to individuals suffering from an increased body burden of oxalate and oxalate-associated disorders.
本研究旨在评估几种广泛应用于益生菌乳制品和药物制剂中的乳酸杆菌的草酸盐降解活性。对嗜酸乳杆菌中的草酰辅酶A脱羧酶和甲酰辅酶A转移酶进行功能表征，以评估乳酸杆菌在调节肠道草酸盐稳态方面的潜在作用。
为了测定属于12个种的60株乳酸杆菌的草酸盐降解能力，采用酶法进行筛选。发现不同种的草酸盐降解能力存在很大差异。嗜酸乳杆菌和加氏乳杆菌菌株表现出最高的草酸盐降解活性。克隆并测序了嗜酸乳杆菌LA14的草酰辅酶A脱羧酶和甲酰辅酶A转移酶基因。通过毛细管电泳评估重组酶的活性。
鉴定出了具有高草酸盐降解活性的乳酸杆菌菌株。证明了嗜酸乳杆菌LA14的草酰辅酶A脱羧酶和甲酰辅酶A转移酶在草酸盐分解代谢中的功能和意义。这些结果表明乳酸杆菌菌株在人体肠道中降解草酸盐的潜在用途。
鉴定具有草酸盐降解活性的益生菌菌株可为患有草酸盐体内负荷增加和草酸盐相关疾病的个体提供这种能力。
