Radwan S S, Al-Hasan R H, Mahmoud H M, Eliyas M
Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait.
J Appl Microbiol. 2007 Dec;103(6):2160-7. doi: 10.1111/j.1365-2672.2007.03454.x. Epub 2007 Oct 22.
The objectives were to count and identify the oil-utilizing bacteria associated with fish, and to study their hydrocarbon-degradation potential.
The standard dilution-plate method using a medium with crude oil as a sole source of carbon and energy revealed that 10 different fish sorts from the Arabian Gulf and two from fish farms accommodated millions of oil-utilizing bacteria per square centimetre of fish surface and per gram of gills and guts. According to their 16S rRNA sequences, those bacteria were affiliated to Psychrobacter, Vibrio, Planococcus, Pseudomonas and Actinobacterium. Planktonic and benthic biomass samples from the Gulf were also rich in oil-utilizing bacteria, but with different composition. All isolates could grow on n-alkanes from C(8) to C(40) and three representative aromatics as individual sole sources of carbon and energy. Quantitative analysis of hydrocarbons by gas-liquid chromatography revealed that the biomass samples of the individual bacteria could consume crude oil, n-octadecane and phenanthrene in liquid media.
The abundant oil-utilizing bacterial associated with fish have the potential for cleaning oily waters.
Aquatic fauna accommodates rich consortia of oil-utilizing bacteria.
目标是对与鱼类相关的利用石油的细菌进行计数和鉴定，并研究它们的碳氢化合物降解潜力。
使用以原油作为唯一碳源和能源的培养基的标准稀释平板法显示，来自阿拉伯湾的10种不同鱼类以及来自养鱼场的2种鱼类，每平方厘米鱼表面、每克鳃和肠道中容纳数百万利用石油的细菌。根据它们的16S rRNA序列，这些细菌隶属于嗜冷杆菌属、弧菌属、动性球菌属、假单胞菌属和放线菌属。来自海湾的浮游生物和底栖生物样本中也富含利用石油的细菌，但组成不同。所有分离株都能以C(8)至C(40)的正构烷烃和三种代表性芳烃作为单独的唯一碳源和能源生长。通过气液色谱法对碳氢化合物进行定量分析表明，单个细菌的生物量样本能够在液体培养基中消耗原油、正十八烷和菲。
与鱼类相关的丰富的利用石油的细菌具有清洁含油水域的潜力。
水生动物群中容纳着丰富的利用石油的细菌群落。
