Khazen D, Jendoubi-Ayed S, Gorgi Y, Sfar I, Abderrahim E, Ben Abdallah T, Ayed K
Laboratoire de recherche Immunologie de la transplantation rénale et Immuno-pathologie, Laboratoire d'Immunologie EPS Charles Nicolle, Boulevard du 9 Avril, 1006 Tunis, Tunisia.
Transplant Proc. 2007 Oct;39(8):2563-4. doi: 10.1016/j.transproceed.2007.08.031.
Acute rejection is the main cause of early renal allograft failure. Adhesion molecules provide signals for activation and recruitment of effector cells leading to graft infiltration by host T cells, which are key to allograft rejection. This study was undertaken to analyze the adhesion molecule gene polymorphisms in renal transplant recipients and to investigate their potential association with the development of acute allograft rejection. A total of 120 renal transplant recipients and 100 controls were retrospectively genotyped. Seven nucleotide polymorphisms in intracellular adhesion molecule (ICAM)-1, platelet endothelial cell adhesion molecule (PECAM)-1, L-selectin, and E-selectin were analyzed using allele-specific polymerase chain reaction (PCR)-SSP assay and PCR-restriction fragment length polymorphism (RFLP). Recipients were selected on the basis of the development of acute allograft rejection in the first 6 months after renal transplantation. Forty-one patients developed acute allograft rejection and 79 showed uneventful courses. There was no evidence for an association of any polymorphism with acute rejection. Thus, we concluded that these genes do not predispose to acute renal allograft rejection.
急性排斥反应是早期肾移植失败的主要原因。黏附分子为效应细胞的激活和募集提供信号，导致宿主T细胞浸润移植肾，而宿主T细胞是同种异体移植排斥反应的关键因素。本研究旨在分析肾移植受者黏附分子基因多态性，并探讨其与急性移植排斥反应发生的潜在关联。对120例肾移植受者和100例对照者进行回顾性基因分型。采用等位基因特异性聚合酶链反应（PCR）-序列特异性引物（SSP）分析和PCR-限制性片段长度多态性（RFLP）方法，分析细胞间黏附分子（ICAM）-1、血小板内皮细胞黏附分子（PECAM）-1、L-选择素和E-选择素的7个核苷酸多态性。根据肾移植后前6个月内急性移植排斥反应的发生情况选择受者。41例患者发生急性移植排斥反应，79例病程顺利。没有证据表明任何多态性与急性排斥反应有关。因此，我们得出结论，这些基因不会导致急性肾移植排斥反应。
