Krichen H, Sfar I, Jendoubi-Ayed S, Makhlouf M, Ben Rhomdhane T, Bardi R, Aouadi H, Ben Abdallah T, Ayed K, Gorgi Y
Laboratory of Immunology, Charles Nicolle Hospital, Tunis, Tunisia.
Transplant Proc. 2009 Oct;41(8):3305-7. doi: 10.1016/j.transproceed.2009.08.032.
CTLA-4 and CD28 are T lymphocyte receptors involved in the regulation of T-cell activation. Allograft rejection is an alloimune response which is strongly dependent on T-cell proliferation. Thus, we examined the relationship between CTLA-4 and CD28 gene polymorphisms and renal transplant outcomes. We genotyped 141 renal recipients and 229 healthy controls using PCR-SSP methods for the (-318) C/T polymorphism in the promoter region of the CTLA-4 gene and IVS3 (+17) T/C on intron 3 of the CD28 gene, and by PCR-RFLP method for exon 1 (+49) A/G and CT60 G/A within the 3'-untranslated region (UTR) of the CTLA-4 gene. Patients were classified into two groups: Group I included 23 HLA-identical haplotype allograft recipients and group II, 118 recipients with one or more mismatches in HLA haplotypes. Thirty-six patients developed at least one acute rejection episode (ARE). No significant differences were observed between the genotypes or the allele distribution between ARE and non-ARE patients. However, in group I, (+49) A and CT60 (G) allele frequencies were lower in patients with ARE than those without ARE (0.100 and 0.400 vs 0.361 and 0.722 respectively). However, the difference was not significant. Our study suggested that these alleles may confer protection against renal allograft loss.
CTLA-4和CD28是参与T细胞活化调节的T淋巴细胞受体。同种异体移植排斥是一种强烈依赖于T细胞增殖的同种免疫反应。因此，我们研究了CTLA-4和CD28基因多态性与肾移植结果之间的关系。我们采用PCR-SSP方法对141例肾移植受者和229例健康对照进行基因分型，检测CTLA-4基因启动子区（-318）C/T多态性以及CD28基因第3内含子IVS3（+17）T/C多态性，并采用PCR-RFLP方法检测CTLA-4基因3'-非翻译区（UTR）中外显子1（+49）A/G和CT60 G/A多态性。患者分为两组：第一组包括23例HLA单倍型相同的同种异体移植受者，第二组包括118例HLA单倍型有一个或多个错配的受者。36例患者发生了至少一次急性排斥反应（ARE）。ARE患者与未发生ARE患者的基因型或等位基因分布之间未观察到显著差异。然而，在第一组中，发生ARE的患者中（+49）A和CT60（G）等位基因频率低于未发生ARE的患者（分别为0.100和0.400，而0.361和0.722）。然而，差异不显著。我们的研究表明，这些等位基因可能对预防肾移植失败具有保护作用。
