Morikawa Rei, Tsujimoto Masafumi, Arai Hiroyuki, Aoki Junken
Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Methods Enzymol. 2007;434:1-13. doi: 10.1016/S0076-6879(07)34001-9.
Although a number of phospholipase A(1)s (PLA(1)s) have been identified in the recent decade, the physiological functions of PLA(1)s remain almost elusive. The major reason for this is the poor availability of assay methods. In many studies, radiolabeled phospholipid substrates have been used to measure PLA(1) activity. This chapter describes the conventional PLA(1) assay using a radiolabeled substrate and a novel PLA(1) assay using electrospray ionization mass spectrometry.
尽管近十年来已鉴定出多种磷脂酶A(1)（PLA(1)），但其生理功能仍几乎不为人知。主要原因是检测方法不足。在许多研究中，放射性标记的磷脂底物已被用于测量PLA(1)活性。本章介绍了使用放射性标记底物的传统PLA(1)检测方法以及使用电喷雾电离质谱的新型PLA(1)检测方法。
