Postle Anthony D, Wilton David C, Hunt Alan N, Attard George S
School of Medicine, University of Southampton, Southampton SO17 1BJ, UK.
Prog Lipid Res. 2007 May-Jul;46(3-4):200-24. doi: 10.1016/j.plipres.2007.04.001. Epub 2007 Apr 24.
Recent advances in electrospray ionisation mass spectrometry (ESI-MS) have greatly facilitated the analysis of phospholipid molecular species in a growing diversity of biological and clinical settings. The combination of ESI-MS and metabolic labelling employing substrates labelled with stable isotopes is especially exciting, permitting studies of phospholipid synthesis and turnover in vivo. This review will first describe the methodology involved and will then detail dynamic lipidomic studies that have applied the stable isotope incorporation approach. Finally, it will summarise the increasing number of studies that have used ESI-MS to characterise structural and signalling phospholipid molecular species in development and disease.
电喷雾电离质谱（ESI-MS）的最新进展极大地推动了在越来越多的生物和临床环境中对磷脂分子种类的分析。ESI-MS与采用稳定同位素标记底物的代谢标记相结合尤其令人兴奋，它使体内磷脂合成和周转的研究成为可能。本综述将首先描述所涉及的方法，然后详细介绍应用稳定同位素掺入方法的动态脂质组学研究。最后，它将总结越来越多使用ESI-MS来表征发育和疾病中结构和信号磷脂分子种类的研究。
