探索RecA-DNA细丝的结构。荧光鸟嘌呤类似物的优势。
Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog.
作者信息
Singleton Scott F, Roca Alberto I, Lee Andrew M, Xiao Jie
机构信息
Division of Medicinal Chemistry & Natural Products, School of Pharmacy, The University of North Carolina at Chapel Hill, CB 7360, Chapel Hill, NC 27599-7360, USA.
出版信息
Tetrahedron. 2007 Apr 23;63(17):3553-3566. doi: 10.1016/j.tet.2006.10.092.
Abstract
The RecA protein of Escherichia coli plays a crucial roles in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.
摘要
大肠杆菌的RecA蛋白在DNA重组与修复以及细菌致病性的各个方面都发挥着关键作用。RecA-ATP-ssDNA复合物的形成启动了所有RecA活性，然而，对这种细丝的完整结构和机制描述仍然难以捉摸。使用荧光标记的寡核苷酸对RecA-DNA相互作用进行了分析。对荧光素和几种荧光核苷进行了直接比较。荧光鸟嘌呤类似物6-甲基异黄蝶呤（6MI）显示出优于其他荧光团的显著优势，是表征RecA-DNA相互作用的重要新工具。
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