Wall M J, Declusin R J, Soergel K H, Baker R D
Biochim Biophys Acta. 1976 May 21;433(3):654-61. doi: 10.1016/0005-2736(76)90288-1.
Short chain fatty acids suddenly produce a phasic increase in transmural electrical potential difference (PD) when placed in the lumen of rat small intestine in vivo. With concentrations of propionate ranging from 50 muM to 1000 muM the amplitude of the response in jejunum is about 5.5 mV. The concentration giving half this effect is about 20 muM. With 10 mM propionate the duration of the response is 3-5 min; after this, PD again equals the control value and the gut is refractory to further additions. Removing propionate from the mucosal surface produces no change in PD, but does restore responsiveness to subsequent exposure to short chain fatty acids. This effect is independent of a variety of other alterations in PD such as those caused by sugars, amino acids, bile salts, theophylline, prostaglandins, and ATP. Mechanism and significance of this surprisingly sensitive response remain obscure.
短链脂肪酸在体内置于大鼠小肠肠腔时，会突然使跨壁电势差（PD）产生阶段性增加。当丙酸浓度在50μM至1000μM范围内时，空肠的反应幅度约为5.5mV。产生此效应一半时的浓度约为20μM。使用10mM丙酸时，反应持续时间为3 - 5分钟；在此之后，PD再次等于对照值，肠道对进一步添加物产生不应性。从黏膜表面去除丙酸不会使PD发生变化，但确实会恢复对随后暴露于短链脂肪酸的反应性。这种效应独立于PD的多种其他变化，例如由糖、氨基酸、胆汁盐、茶碱、前列腺素和ATP引起的变化。这种惊人的敏感反应的机制和意义仍不清楚。
