Ye Wei, Huang Dong-Sheng, Chen Wei-Jian, Li Chun-Hai, Peng Yan, Liang An-Jing, Liu Shang-Li
Department of Orthopedics, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Oct;27(10):1485-8.
To investigate the association of 86 bp variable number tandem repeat (VNTR) polymorphism of interleukin-1 receptor antagonist (IL-1Ra) gene with lumbar disc disease and intervertebral disc degeneration.
The 86 bp VNTR polymorphism of IL-1Ra gene was analyzed with PCR and electrophoresis for 81 patients with lumbar disc disease and 101 volunteers without sciatica (control). The grade of intervertebral disc degeneration was determined with magnetic resonance imaging, and the association of 86 bp VNTR polymorphisms with lumbar disc disease and intervertebral disc degeneration in those younger than 45 years was assessed.
The presence of 86bp VNTR polymorphisms of IL-1Ra gene was detected in both patients with lumbar disc disease and the control subjects. The distribution of 86 bp VNTR polymorphisms of IL-1Ra gene showed no significant difference between the two groups, but the distributions of 1/1, 1/2 and 2/2 or 1, 2 genotypes differed significantly. The current data did not support a significant association between the distribution of IL-1Ra gene 86bp VNTR polymorphism and lumbar disc degeneration.
IL-1Ra gene 86bp VNTR polymorphism is present among Chinese population in association with lumbar disc disease, but not with lumbar disc degeneration.
探讨白细胞介素-1受体拮抗剂（IL-1Ra）基因86bp可变数目串联重复序列（VNTR）多态性与腰椎间盘疾病及椎间盘退变的关系。
采用聚合酶链反应（PCR）和电泳技术分析81例腰椎间盘疾病患者及101例无坐骨神经痛志愿者（对照组）的IL-1Ra基因86bp VNTR多态性。通过磁共振成像确定椎间盘退变程度，并评估45岁以下人群中86bp VNTR多态性与腰椎间盘疾病及椎间盘退变的关系。
在腰椎间盘疾病患者和对照组中均检测到IL-1Ra基因86bp VNTR多态性。两组间IL-1Ra基因86bp VNTR多态性的分布无显著差异，但1/1、1/2和2/2或1、2基因型的分布存在显著差异。目前的数据不支持IL-1Ra基因86bp VNTR多态性分布与椎间盘退变之间存在显著关联。
中国人群中存在IL-1Ra基因86bp VNTR多态性，其与腰椎间盘疾病相关，但与椎间盘退变无关。
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