Tumer Mehmet Kemal, Nursal Ayse Feyda, Tekcan Akin, Yerliyurt Kaan, Geyko Anastasia, Yigit Serbulent
Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Gaziosmanpasa University, Tokat, Turkey.
Faculty of Medicine, Department of Medical Biology, Gaziosmanpasa University, Tokat, Turkey.
J Clin Lab Anal. 2018 Feb;32(2). doi: 10.1002/jcla.22255. Epub 2017 Jun 14.
Temporomandibular joint disorders (TMD) are a group of disorders involving temporomandibular joint and related structures. Interleukine-1 receptor antagonist (IL-1Ra) is an important anti-inflammatory molecule that competes with other interleukin-1 molecules. This study was designed to investigate the possible association of the IL-1Ra VNTR variant with the risk of TMD in the Turkish population.
Peripheral blood samples were collected from 100 patients with TMD (23 males, 77 females) and 110 healthy individuals (35 males, 75 females). Genotyping of IL-1Ra 86 bp VNTR variant was evaluated by gel electrophoresis after polymerase chain reaction (PCR).
Our results show that there is a statistically significant difference between TMD patients and control group with respect to IL-1Ra genotype distribution and allele frequencies. 1.2, 1.4, and 4.4 genotypes were more common in patients, while 2.2 and 3.3 genotypes were rarer (P<.000). Frequency of alleles 1 and 4 was higher in patient groups (P<.000), whereas alleles 2 and 3 had a lower frequency in patients with TMD (P<.000).
This is the first correlation study that evaluates the association between IL-1Ra gene VNTR variant and TMD. The VNTR variant related to IL-1Ra gene showed a strong pattern of association with TMD that may have a potential impact on disease counseling and management. Larger studies with various ethnicities are needed to establish the impact of IL-1Ra VNTR variant on risk of developing TMD.
颞下颌关节紊乱病（TMD）是一组累及颞下颌关节及相关结构的疾病。白细胞介素-1受体拮抗剂（IL-1Ra）是一种重要的抗炎分子，可与其他白细胞介素-1分子竞争。本研究旨在调查土耳其人群中IL-1Ra VNTR变异与TMD风险之间的可能关联。
收集100例TMD患者（23例男性，77例女性）和110名健康个体（35例男性，75例女性）的外周血样本。聚合酶链反应（PCR）后通过凝胶电泳评估IL-1Ra 86 bp VNTR变异的基因分型。
我们的结果显示，TMD患者与对照组在IL-1Ra基因型分布和等位基因频率方面存在统计学显著差异。1.2、1.4和4.4基因型在患者中更为常见，而2.2和3.3基因型较为罕见（P<0.000）。患者组中等位基因1和4的频率较高（P<0.000），而TMD患者中等位基因2和3的频率较低（P<0.000）。
这是第一项评估IL-1Ra基因VNTR变异与TMD之间关联的相关性研究。与IL-1Ra基因相关的VNTR变异显示出与TMD的强关联模式，这可能对疾病咨询和管理产生潜在影响。需要进行更大规模的不同种族研究，以确定IL-1Ra VNTR变异对TMD发病风险的影响。
