Lu H-K, Chou H-P, Li C-L, Wang M-Y, Wang L-F
Periodontal Clinic of the Dental Department, Taipei Medical University Hospital, Taipei, Taiwan.
J Dent Res. 2007 Nov;86(11):1100-4. doi: 10.1177/154405910708601115.
The purpose of this study was to clarify the main contributory factor of nifedipine-induced gingival overgrowth either by Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) or interleukin-1beta (IL-1beta). Human gingival fibroblasts from healthy tissues and nifedipine-induced gingival overgrowth tissues were stimulated with nifedipine, IL-1beta, Escherichia coli lipopolysaccharide (Ec-LPS), and Pg-LPS, and the gene expressions were analyzed by RT-PCR. Analysis of the data showed no strong evidence of a synergistic effect of nifedipine and Pg-LPS on IL-6, connective tissue growth factor (CTGF), and type 1 collagen gene expression of either healthy cells or nifedipine-induced gingival overgrowth cells. Among the three stimulants--IL-1beta, Pg-LPS, and Ec-LPS--androgen receptor and IL-6 gene expressions in both the healthy and nifedipine-induced gingival overgrowth groups were strongly up-regulated by the presence of IL-1beta only. Furthermore, the responses to IL-1beta in the nifedipine-induced gingival overgrowth group were stronger than those of the healthy group. It can be concluded that IL-1beta is an important mediator responsible for the higher IL-6 and androgen receptor expression of nifedipine-induced gingival overgrowth cells.
本研究的目的是阐明牙龈卟啉单胞菌脂多糖（Pg-LPS）或白细胞介素-1β（IL-1β）是否为硝苯地平诱导牙龈过度增生的主要促成因素。用硝苯地平、IL-1β、大肠杆菌脂多糖（Ec-LPS）和Pg-LPS刺激来自健康组织和硝苯地平诱导牙龈过度增生组织的人牙龈成纤维细胞，并通过逆转录聚合酶链反应（RT-PCR）分析基因表达。数据分析显示，没有强有力的证据表明硝苯地平和Pg-LPS对健康细胞或硝苯地平诱导牙龈过度增生细胞的白细胞介素-6（IL-6）、结缔组织生长因子（CTGF）和I型胶原基因表达有协同作用。在白细胞介素-1β、Pg-LPS和Ec-LPS这三种刺激物中，仅白细胞介素-1β的存在就强烈上调了健康组和硝苯地平诱导牙龈过度增生组中的雄激素受体和白细胞介素-6基因表达。此外，硝苯地平诱导牙龈过度增生组对白细胞介素-1β的反应比健康组更强。可以得出结论，白细胞介素-1β是导致硝苯地平诱导牙龈过度增生细胞中白细胞介素-6和雄激素受体表达升高的重要介质。
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