[利用蛇毒固定化磷酸二酯酶分析寡脱氧核糖核苷酸的核苷酸组成]
[Use of snake venom immobilized phosphodiesterase for analysis of the nucleotide composition of oligodesoxyribonucleotides].
作者信息
Kagramanova V K, Aukati M F, Fedorova G V, Shabarova Z A
出版信息
Biokhimiia. 1976 Feb;41(2):394-7.
PMID:179617
Abstract
A simple one-step technique for the analysis of nucleotide composition of oligodeoxyribonucleotides is described. The method is based on enzymatic hydrolysis and subsequent separation of hydrolysate on a "combined" column with Aminex A 28 anionite, on which top immobilyzed on DEAE cellulose snake venom phosphodiesterase is layed. The method is tested using a number of synthetic di- and tri-deoxyribonucleotides.
摘要
本文描述了一种用于分析寡脱氧核糖核苷酸核苷酸组成的简单一步技术。该方法基于酶促水解，随后将水解产物在装有Aminex A 28阴离子交换剂的“组合”柱上进行分离，在该柱上固定有DEAE纤维素上的蛇毒磷酸二酯酶。使用多种合成二脱氧核糖核苷酸和三脱氧核糖核苷酸对该方法进行了测试。
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