Cherdshewasart Wichai, Sutjit Wandee, Pulcharoen Kade, Panriansaen Rattana, Chulasiri Malyn
Department of Biology, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok 10330, Thailand.
J Ethnopharmacol. 2008 Jan 4;115(1):96-103. doi: 10.1016/j.jep.2007.09.008. Epub 2007 Sep 18.
Mucuna collettii Lace is a Thai herb with a long record of consumption among mature Thai males for the promotion of sexual potency. The mutagenic and antimutagenic potentials of Mucuna collettii extract were carried out by using the Ames test pre-incubation method in the presence and absence of S9 mixture. Salmonella typhimurium strains TA 98 and TA 100 were applied as the tester strains. Prior to mutagenic and antimutagenic tests, the survival of the tester strains was performed by treating with the plant extract. Results showed Mucuna collettii extract exhibited strong cytotoxic effects in a dose-dependent manner. Toxicity of the plant was confirmed in mice in which negative adverse effect was found in kidney, uterus, ovary, and testis. Mucuna collettii extract in the presence and absence of S9 mixture was negative for mutagenic Ames test. Mucuna collettii extract in the presence and absence of S9 mixture was positive for antimutagenic Ames test towards either one or both of the tested mutagens: 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2) and benzo(a)pyrene. The antimutagenic activity of the plant extract was confirmed in rec-assays. Micronucleus test demonstrated that Mucuna collettii extract at high dose and a long incubation time could induce micronucleus formation in tested animals, but less than the response of the positive control. The overall mutagenic and antimutagenic assays are further evidences for the antimutagenic potential of Mucuna collettii.
柯氏黧豆是一种泰国草药，在成熟的泰国男性中有着悠久的食用历史，用于增强性能力。采用艾姆斯试验预孵育法，在有和没有S9混合物的情况下，对柯氏黧豆提取物的致突变和抗突变潜力进行了研究。鼠伤寒沙门氏菌菌株TA 98和TA 100用作测试菌株。在进行致突变和抗突变试验之前，通过用植物提取物处理来检测测试菌株的存活率。结果表明，柯氏黧豆提取物呈现出剂量依赖性的强细胞毒性作用。在小鼠中证实了该植物的毒性，在肾脏、子宫、卵巢和睾丸中未发现负面不良影响。在有和没有S9混合物的情况下，柯氏黧豆提取物的致突变性艾姆斯试验均为阴性。在有和没有S9混合物的情况下，柯氏黧豆提取物对一种或两种受试诱变剂2-(2-呋喃基)-3-(5-硝基-2-呋喃基)-丙烯酰胺(AF-2)和苯并(a)芘的抗突变性艾姆斯试验均为阳性。植物提取物的抗突变活性在重组试验中得到证实。微核试验表明，高剂量和长时间孵育的柯氏黧豆提取物可在受试动物中诱导微核形成，但低于阳性对照的反应。整体的致突变和抗突变试验进一步证明了柯氏黧豆的抗突变潜力。
