吸收光谱、荧光光谱及共振能量转移方法的基本原理。

Basic aspects of absorption and fluorescence spectroscopy and resonance energy transfer methods.

作者信息

Shanker Natasha, Bane Susan L

机构信息

Department of Chemistry, State University of New York at Binghamton, Binghamton, New York 13902, USA.

出版信息

Methods Cell Biol. 2008;84:213-42. doi: 10.1016/S0091-679X(07)84008-8.

DOI:10.1016/S0091-679X(07)84008-8

PMID:17964933

Abstract

Absorption and fluorescence spectroscopy are the heart of life science laboratory techniques. These two spectroscopic tools are sensitive, are easy to use, and can provide a wide range of information. The goal of this chapter is to familiarize the reader with some basic principles and applications of absorption and steady-state fluorescence spectroscopy. Selected applications are illustrated with examples.

摘要

吸收光谱和荧光光谱是生命科学实验室技术的核心。这两种光谱工具灵敏度高、易于使用，并且能够提供广泛的信息。本章的目的是使读者熟悉吸收光谱和稳态荧光光谱的一些基本原理及应用。文中通过实例对选定的应用进行了说明。