神经回路的双光子光刺激与成像

Two-photon photostimulation and imaging of neural circuits.

作者信息

Nikolenko Volodymyr, Poskanzer Kira E, Yuste Rafael

机构信息

Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, Box 2435, New York, New York 10027, USA.

出版信息

Nat Methods. 2007 Nov;4(11):943-50. doi: 10.1038/nmeth1105. Epub 2007 Oct 28.

DOI:10.1038/nmeth1105

PMID:17965719

Abstract

We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.

摘要

我们介绍一种光学方法，通过使用具有光束复用功能的MNI-谷氨酸双光子解笼技术，以任意时空模式刺激脑片上的单个神经元。该方法具有单细胞和三维精度。通过依次刺激多达一千个潜在的突触前神经元，我们生成了一个细胞输入的详细功能图谱。我们将这种方法与双光子钙成像相结合，形成一种全光学方法来成像和操纵神经回路活动。