功能性群体感应系统影响鼠疫耶尔森菌中的生物膜形成和蛋白质表达。
Functional quorum sensing systems affect biofilm formation and protein expression in Yersinia pestis.
作者信息
Bobrov Alexander G, Bearden Scott W, Fetherston Jacqueline D, Khweek Arwa Abu, Parrish Kenneth D, Perry Robert D
机构信息
Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, USA.
出版信息
Adv Exp Med Biol. 2007;603:178-91. doi: 10.1007/978-0-387-72124-8_15.
Gram-negative bacteria predominantly use two types of quorum sensing (QS) systems--LuxI-LuxR, responsible for synthesis of N-acylhomoserine lactones (AHL or AI-1 signal molecule), and LuxS, which makes furanones (AI-2 signal molecule). We showed that LuxS and two LuxI-LuxR (YtbIR and YpsIR) systems are functional in Y. pestis. Four different AHL molecules were detected in Y. pestis extracts using TLC bioassays. Our data suggest that YtbIR is responsible for the production of long chain AHLs. Confocal laser scanning microscopy showed that biofilm formation is decreased in an ytbIR ypsIR luxS mutant. Two-dimensional gel electrophoresis revealed altered levels of protein expression in a Y. pestis triple QS mutant at 26 degrees C and 37 degrees C.
革兰氏阴性菌主要使用两种群体感应（QS）系统——负责合成N-酰基高丝氨酸内酯（AHL或AI-1信号分子）的LuxI-LuxR系统，以及产生呋喃酮（AI-2信号分子）的LuxS系统。我们发现LuxS和两个LuxI-LuxR（YtbIR和YpsIR）系统在鼠疫耶尔森菌中发挥功能。通过薄层层析生物测定法在鼠疫耶尔森菌提取物中检测到四种不同的AHL分子。我们的数据表明YtbIR负责长链AHL的产生。共聚焦激光扫描显微镜显示，ytbIR ypsIR luxS突变体中的生物膜形成减少。二维凝胶电泳显示，鼠疫耶尔森菌三重QS突变体在26℃和37℃时蛋白质表达水平发生改变。
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