Rodríguez Islay, Martínez Raydel, Zamora Yarelys, Rodríguez José Enrique, Fernández Carmen, Obregón Ana Margarita
Instituto de Medicina Tropical Pedro Kouri, Marianao, Ciudad de La Habana.
Rev Cubana Med Trop. 2005 Jan-Apr;57(1):32-7.
A quantitative ELISA for the detection of IgG antibodies in response to the Cuban vaccine against the human leptospirosis (vax-SPIRAL) was applied to 930 volunteers'sera, 483 immunized with vax-SPIRAL and 447 with a vaccine against hepatitis B. Samples were taken before beginning the study, 21 days after the second dose and a year later. A high seroprevalence of antibodies to the vaccine serovars was observed before beginning the study. The initial value of the response doubled at 21 days of the second dose in 45 % of the individuals. There were markedly significant differences (p=0,000000) between both groups. A similar response to the three leptospiral serovars was found. After the year the levels of antibodies decreased; however, 23.1% of the individuals were able to duplicate their level of antibodies regarding the initial moment, and the significant difference with the group that received the vaccine against hepatitis B still existed. The application of this Cuban immunogen amomg the main risk groups was recommended.
一种用于检测针对古巴人钩端螺旋体病疫苗（vax - SPIRAL）产生的IgG抗体的定量酶联免疫吸附测定（ELISA）应用于930名志愿者的血清，其中483人接种了vax - SPIRAL疫苗，447人接种了乙肝疫苗。在研究开始前、第二剂疫苗接种后21天以及一年后采集样本。在研究开始前观察到针对疫苗血清型的抗体具有较高的血清阳性率。45%的个体在第二剂疫苗接种21天时反应的初始值翻倍。两组之间存在显著差异（p = 0.000000）。发现对三种钩端螺旋体血清型有类似反应。一年后抗体水平下降；然而，23.1%的个体能够使抗体水平相对于初始时刻翻倍，并且与接种乙肝疫苗的组仍存在显著差异。建议在主要风险人群中应用这种古巴免疫原。
