Szakiel A, Janiszowska W
Institute of Biochemistry, Warsaw University, Poland.
Acta Biochim Pol. 1991;38(1):47-51.
The transport of oleanolic acid and its glycosides (3-O-monoglucuronide F and 3-O-monoglucoside I) into the vacuoles of C. officinalis leaves was studied. It was found that these monoglycosides are transported into the vacuolar space whereas free oleanolic acid only binds with the tonoplast. The transport of monoglycosides depends on pH of the medium, the optimum pH for monoglucoside I and monoglucuronide F being 6.0 and 7.0, respectively. Moreover, it was demonstrated that the transport of monoglucoside I, in contrast to that of monoglucuronide F, depends on ATP at 0.5-2.0 mM concentrations. The presented results indicate that different mechanisms underlie the transport of these two compounds into vacuoles.
研究了齐墩果酸及其糖苷（3 - O - 单葡萄糖醛酸苷F和3 - O - 单葡萄糖苷I）向药用蒲公英叶片液泡中的转运。发现这些单糖苷被转运到液泡空间，而游离的齐墩果酸仅与液泡膜结合。单糖苷的转运取决于培养基的pH值，单葡萄糖苷I和单葡萄糖醛酸苷F的最佳pH值分别为6.0和7.0。此外，还证明了与单葡萄糖醛酸苷F相比，单葡萄糖苷I的转运在0.5 - 2.0 mM浓度下依赖于ATP。所呈现的结果表明这两种化合物向液泡转运的机制不同。
