[大鼠甲状腺实验性肿瘤可溶性部分中各种蛋白激酶及部分共纯化内源性底物的存在情况]
[Presence of various protein kinases and of partially copurified endogenous substrates in the soluble fraction of an experimental tumor of the rat thyroid gland].
作者信息
Meldolesi M F, Laccetti P, Macchia V
出版信息
C R Seances Soc Biol Fil. 1975;169(6):1448-54.
PMID:179675
Abstract
Various fractions containing protein-kinases have been partly purified from supernatant (105 000 g) of an experimental rat's thyroid cancer (1-8 Wollman). These fractions are activated at different levels by cyclic nucleotides and present an unequal affinity for protamine; they phosphorylate an endogenous substrate apparently copurified with the enzyme fractions and adorbed with them on calcium phosphate gel.
摘要
已从实验性大鼠甲状腺癌（1-8号沃曼）的上清液（105000克）中部分纯化出了各种含蛋白激酶的组分。这些组分被环核苷酸以不同程度激活，对鱼精蛋白的亲和力也不相同；它们能使一种显然与酶组分共纯化并与它们一起吸附在磷酸钙凝胶上的内源性底物发生磷酸化。
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