Chang Hui-Yun, Du Jun-Zheng, Cong Guo-Zheng, Shao Jun-Jun, Lin Tong, Xie Qing-Ge
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Bing Du Xue Bao. 2007 Sep;23(5):407-11.
The full-length genomic sequence of foot-and-mouth disease virus (FMDV) Asia1/YNBS/58 strain was determined by RT-PCR and compared with other 17 reference strains. The results showed that the complete genome of Asia1/YNBS/58 was 8164nt long including a 1061-nt 5' untranslated region (UTR), a 6990-nt open reading frame (ORF), and a 113-nt 3'UTR. The homology analysis indicated that the UTR regions and non-structural proteins were more conserved than the structural proteins in FMDV. VP1 exhibited the lowest conservation and VP4 was exceptionally conserved. The VP1-, VP2-, and VP3-based phylogenetic trees were divided into distinct clusters according to different serotypes, while the other gene-based phylogenetic trees exhibited some degree of intercross among serotypes. This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.
通过逆转录聚合酶链反应（RT-PCR）测定了口蹄疫病毒（FMDV）Asia1/YNBS/58株的全长基因组序列，并与其他17个参考毒株进行了比较。结果显示，Asia1/YNBS/58的完整基因组长度为8164nt，包括一个1061nt的5'非翻译区（UTR）、一个6990nt的开放阅读框（ORF）和一个113nt的3'UTR。同源性分析表明，FMDV的UTR区域和非结构蛋白比结构蛋白更保守。VP1的保守性最低，而VP4异常保守。基于VP1、VP2和VP3构建的系统发育树根据不同血清型分为不同的簇，而基于其他基因构建的系统发育树在血清型之间表现出一定程度的交叉。本研究首次描述了中国Asia1血清型FMDV的全长基因组序列。
