Cada Zdenek, Boucek Jan, Dvoranková Barbora, Chovanec Martin, Plzák Jan, Kodets Roman, Betka Jan, Pinot Gian L, Gabius Hans-Joachim, Smetana Karel
Institute of Anatomy and Head and Neck Surgery, First Faculty of Medicine, Second Faculty of Medicine, Charles University, Prague, Czech Republic.
Anticancer Res. 2007 Sep-Oct;27(5A):3279-84.
This study presents initial data on presence of nucleostemin--a nucleolar protein typical of stem cells in the normal squamous epithelium of the oropharynx and larynx - in squamous cell carcinoma originating from these epithelia.
Differentiation and proliferation markers such as keratins, beta-catenin, galectin-1, and Ki-67 were studied in parallel with nucleostemin for defining cell characteristics.
Nucleostemin was detected in nucleoli of both proliferating basal cells and terminally differentiated suprabasal cells of normal epithelium and in tumor cells. Importantly, malignant transformation was connected with a significant enlargement of nucleostemin-positive nucleoli in these cell types.
Detection of nucleostemin in head and neck cancer cells, together with the size of nucleoli, may be important in the evaluation of tumor differentiation and biology.
本研究提供了关于核干细胞素存在情况的初始数据，核干细胞素是一种在口咽和喉正常鳞状上皮中典型的干细胞核仁蛋白，存在于源自这些上皮的鳞状细胞癌中。
将角蛋白、β-连环蛋白、半乳糖凝集素-1和Ki-67等分化和增殖标志物与核干细胞素并行研究，以确定细胞特征。
在正常上皮的增殖基底细胞和终末分化的基底上层细胞的核仁以及肿瘤细胞中均检测到核干细胞素。重要的是，在这些细胞类型中，恶性转化与核干细胞素阳性核仁的显著增大有关。
头颈部癌细胞中核干细胞素的检测以及核仁大小，可能对评估肿瘤分化和生物学特性具有重要意义。
