Uchimura Shunji, Iizuka Norio, Tamesa Takao, Miyamoto Takanobu, Hamamoto Yoshihiko, Oka Masaaki
Department of Computer Science and Systems Engineering, Faculty of Engineering, Yamaguchi University, Yamaguchi, Japan.
Anticancer Res. 2007 Sep-Oct;27(5A):3323-30.
The majority of hepatocellular carcinomas (HCCs) correlated with infection by either hepatitis B or C virus (HBV or HCV) showing various geographic distributions, making it impossible to identify common gene signatures responsible for HCC recurrence. In this study we performed in silico resampling analysis of DNA microarray data that can reproduce virtually the geographic distribution pattern of HBV and HCV in 6 representative geographic regions. With the use of the Fisher ratio, genes associated with early intrahepatic recurrence of HCC within 1 year of surgery were identified in the 6 geographic virtual cohorts, each consisting of 1,000 virtual samples. The top 100 genes among each virtual cohort were compared. Many human leukocyte antigen (HLA) family genes were common among the 6 geographic virtual cohorts, suggesting that this gene family represents the pathway most responsible for early intrahepatic recurrence of HCC worldwide. This resampling approach is useful for identifying common pathways involved in various aspects of HCC.
大多数肝细胞癌（HCC）与乙型肝炎或丙型肝炎病毒（HBV或HCV）感染相关，呈现出不同的地理分布，这使得难以确定导致HCC复发的共同基因特征。在本研究中，我们对DNA微阵列数据进行了计算机重采样分析，该分析能够再现6个代表性地理区域中HBV和HCV的地理分布模式。利用费舍尔比率，在6个地理虚拟队列中识别出与术后1年内HCC早期肝内复发相关的基因，每个队列由1000个虚拟样本组成。比较了每个虚拟队列中的前100个基因。许多人类白细胞抗原（HLA）家族基因在6个地理虚拟队列中是共有的，这表明该基因家族代表了全球范围内HCC早期肝内复发最主要的途径。这种重采样方法有助于识别HCC各个方面所涉及的共同途径。
