乙醇对有酒精中毒风险儿童的红细胞膜的流化作用。

Ethanol's fluidizing effects on RBC membranes from children at risk for alcoholism.

作者信息

Hill S Y, Zubenko G S, Gronlund S, Teply I

机构信息

Alcoholism and Genetics Research Program, University of Pittsburgh School of Medicine, PA 15213.

出版信息

Alcohol. 1991 Sep-Oct;8(5):405-7. doi: 10.1016/0741-8329(91)90678-p.

DOI:10.1016/0741-8329(91)90678-p

PMID:1797036

Abstract

The fluidizing effects of ethanol on membrane fluidity at 37 degrees C have been assessed using steady-state anisotropy measurements in both children at high risk for developing alcoholism and control children. Erythrocyte membranes were labeled with 1,6-diphenyl-1,3,5-hexatriene (DPH) and fluidity measurements recorded by steady-state fluorescence anisotropy for three ethanol concentrations--0, 0.5 M, and 1.0 M. The in vitro fluidizing effects of acute ethanol treatment by concentration were clearly shown in both groups. However, RBC membranes taken from children at high risk for developing alcoholism showed no significant differences in baseline membrane fluidity or to the fludizing effects of ethanol.

摘要

通过稳态各向异性测量，已对有酗酒高风险儿童和对照儿童在37摄氏度下乙醇对膜流动性的流化作用进行了评估。用1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）标记红细胞膜，并通过稳态荧光各向异性记录三种乙醇浓度（0、0.5 M和1.0 M）下的流动性测量结果。两组均清楚显示了急性乙醇处理按浓度产生的体外流化作用。然而，取自有酗酒高风险儿童的红细胞膜在基线膜流动性或对乙醇的流化作用方面均未显示出显著差异。