Plásek J, Cermáková D, Jarolím P
Institute of Physics of the Charles University, Prague, Czechoslovakia.
Biochim Biophys Acta. 1988 Jun 22;941(2):119-22. doi: 10.1016/0005-2736(88)90171-x.
Membranes of intact erythrocytes were labeled by the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) using an improved labeling procedure described previously (Plásek, J. and Jarolím, P. (1987) Gen. Physiol. Biophys. 6, 425-437). The relationship between the steady-state DPH fluorescence anisotropy r and the mean corpuscular hemoglobin concentration (MCHC) was studied. Fluorescence anisotropy increased with increasing MCHC. A linear dependence of r = 0.0026 (MCHC) + 0.113 was obtained which enabled us to measure the fluidity of intact red cell membranes. Without this correction for fluorescence quenching by hemoglobin, incorrect conclusions about membrane fluidity could be made. This fact is demonstrated in a group of pyruvate kinase deficient patients compared with a group of healthy blood donors.
使用先前描述的改进标记程序（Plásek, J.和Jarolím, P. (1987) Gen. Physiol. Biophys. 6, 425 - 437），用荧光探针1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）标记完整红细胞的膜。研究了稳态DPH荧光各向异性r与平均红细胞血红蛋白浓度（MCHC）之间的关系。荧光各向异性随MCHC的增加而增加。得到了r = 0.0026（MCHC）+ 0.113的线性关系，这使我们能够测量完整红细胞膜的流动性。如果不进行这种针对血红蛋白荧光猝灭的校正，可能会得出关于膜流动性的错误结论。这一事实在一组丙酮酸激酶缺乏症患者与一组健康献血者的比较中得到了证明。
