[乙肝病毒大蛋白（LHBs）在乙型肝炎诊断中的评估及临床意义]

[Evaluation and clinical significance of HBV large protein (LHBs) in diagnosis of hepatitis B].

作者信息

Chen Yong-Jian, Zhou Yong-Lie, Xia Jun, Xia Wei-Wei

机构信息

Clinical Laboratory Center of Zhejiang Provincial People's Hospital, Hangzhou, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Sep;21(3):241-3.

PMID:17971933

Abstract

OBJECTIVE

To explore the significance of HBV large protein (LHBs) in diagnosis of hepatitis B, we detected the LHBs, HBV DNA, PreS1 and other hepatitis B viral markers (HBV M) in the serum of patients infected with HBV.

METHODS

HBV DNA was quantitatively detected using RT-PCR, LHBs, PreS1 and HBV M were analyzed by ELISA in totally 385 serum samples.

RESULTS

There was a significant difference between the positive rate of LHBs (86.97%) and PreS1 (49.5%) in the 307 serum positive for HBV DNA (P less than 0.05). There was a correlation between the levels of LHBs and the logarithm of HBV DNA (r=0.935). In the serum specimens of patients negative for HBeAg, there was no significant difference between the positive rate of LHBs (76.92%) and the HBV DNA (67.95%), but the positive rate of PreS1 (45.73%) was lower than that of LHBs or HBV DNA.

CONCLUSION

There was a close correlation between the copies of HBV DNA and the levels of LHBs, both the positive rate and the coincidence rate of LBHs and HBV DNA were higher than those of PreS1. LHBs can reflect the replication status of HBV.

摘要

目的

为探讨乙肝病毒大蛋白（LHBs）在乙型肝炎诊断中的意义，我们检测了乙肝病毒感染者血清中的LHBs、乙肝病毒脱氧核糖核酸（HBV DNA）、前S1及其他乙肝病毒标志物（HBV M）。

方法

采用逆转录聚合酶链反应（RT-PCR）定量检测HBV DNA，采用酶联免疫吸附测定（ELISA）分析385份血清样本中的LHBs、前S1及HBV M。

结果

在307份HBV DNA阳性的血清中，LHBs阳性率（86.97%）与前S1阳性率（49.5%）之间存在显著差异（P<0.05）。LHBs水平与HBV DNA对数之间存在相关性（r=0.935）。在HBeAg阴性患者的血清标本中，LHBs阳性率（76.92%）与HBV DNA阳性率（67.95%）之间无显著差异，但前S1阳性率（45.73%）低于LHBs或HBV DNA阳性率。