内毒素可诱导嗜酸性粒细胞在大鼠胸腔内积聚。

Endotoxin induces eosinophil accumulation in the rat pleural cavity.

作者信息

Bozza P T, Castro-Faria-Neto H C, Pires A L, Silva P M, Martins M A, Cordeiro R S

机构信息

Departamento de Fisiologia e Farmacodinâmica, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.

出版信息

Braz J Med Biol Res. 1991;24(9):957-60.

PMID:1797292

Abstract

Changes in eosinophil counts after intrathoracic (it) injection of endotoxin (LPS) were investigated in Wistar rats (150-180 g). Increasing doses of endotoxin (62.5-500 ng/cavity) induced a dose-dependent increase in the number of eosinophils recovered from the rat pleural cavity 24 h later. The eosinophilia was apparent within 24 h, peaked within 48 h (from 0.76 +/- 0.12 to 3.68 +/- 0.51 eosinophils x 10(6)/cavity, P less than 0.001) and returned to basal levels 120 h after the it injection of endotoxin (250 ng/cavity). Endotoxin (3 ng-4 micrograms/ml) failed to attract eosinophils in vitro under conditions in which PAF-acether induced a dose-related response. These findings indicate that endotoxin-induced eosinophil migration in vivo is mediated by a secondary mechanism.

摘要

在150 - 180克的Wistar大鼠中，研究了胸腔内注射内毒素（LPS）后嗜酸性粒细胞计数的变化。递增剂量的内毒素（62.5 - 500纳克/腔）导致24小时后从大鼠胸腔回收的嗜酸性粒细胞数量呈剂量依赖性增加。嗜酸性粒细胞增多在24小时内明显，在48小时内达到峰值（从0.76±0.12至3.68±0.51个嗜酸性粒细胞×10⁶/腔，P<0.001），并在内毒素胸腔注射（250纳克/腔）120小时后恢复到基础水平。在内毒素（3纳克 - 4微克/毫升）存在的情况下，血小板活化因子 - 乙醚能诱导剂量相关反应，但内毒素在体外未能吸引嗜酸性粒细胞。这些发现表明，内毒素在体内诱导的嗜酸性粒细胞迁移是由一种次级机制介导的。