Zietkowski Ziemowit, Skiepko Roman, Tomasiak Maria M, Bodzenta-Lukaszyk Anna
Department of Allergology and Internal Medicine, Medical University of Bialystok, Poland.
Respir Res. 2007 Oct 31;8(1):76. doi: 10.1186/1465-9921-8-76.
Exercise-induced bronchoconstriction (EIB) is a highly prevalent condition, whose pathophysiology is not well understood. Endothelins are proinflammatory, profibrotic, broncho- and vasoconstrictive peptides which play an important role in the development of airway inflammation and remodeling in asthma. The aim of the study was to evaluate the changes in endothelin-1 levels in exhaled breath condensate following intensive exercise in asthmatic patients.
The study was conducted in a group of 19 asthmatic patients (11 with EIB, 8 without EIB) and 7 healthy volunteers. Changes induced by intensive exercise in the concentrations of endothelin-1 (ET-1) in exhaled breath condensate (EBC) during 24 hours after an exercise challenge test were determined. Moreover, the possible correlations of these measurements with the results of other tests commonly associated with asthma and with the changes of airway inflammation after exercise were observed.
In asthmatic patients with EIB a statistically significant increase in the concentration of ET-1 in EBC collected between 10 minutes and 6 hours after an exercise test was observed. The concentration of ET-1 had returned to its initial level 24 hours after exercise. No effects of the exercise test on changes in the concentrations of ET-1 in EBC in either asthmatic patients without EIB or healthy volunteers were observed. A statistically significant correlation between the maximum increase in ET-1 concentrations in EBC after exercise and either baseline FENO and the increase in FENO or BHR to histamine 24 hours after exercise in the groups of asthmatics with EIB was revealed.
The release of ET-1 from bronchial epithelium through the influence of many inflammatory cells essential in asthma and interactions with other cytokines, may play an important role in increase of airway inflammation which was observed after postexercise bronchoconstriction in asthmatic patients.
运动诱发的支气管收缩（EIB）是一种非常普遍的病症，其病理生理学尚未得到充分理解。内皮素是促炎、促纤维化、支气管和血管收缩肽，在哮喘气道炎症和重塑的发展中起重要作用。本研究的目的是评估哮喘患者剧烈运动后呼出气冷凝物中内皮素-1水平的变化。
该研究在19名哮喘患者（11名患有EIB，8名未患有EIB）和7名健康志愿者中进行。在运动激发试验后24小时内，测定剧烈运动引起的呼出气冷凝物（EBC）中内皮素-1（ET-1）浓度的变化。此外，观察这些测量结果与其他通常与哮喘相关的测试结果以及运动后气道炎症变化之间的可能相关性。
在患有EIB的哮喘患者中，观察到运动试验后10分钟至6小时收集的EBC中ET-1浓度有统计学意义的增加。运动后24小时，ET-1浓度已恢复到初始水平。在未患有EIB的哮喘患者或健康志愿者中，未观察到运动试验对EBC中ET-1浓度变化的影响。在患有EIB的哮喘患者组中，运动后EBC中ET-1浓度的最大增加与基线呼出气一氧化氮（FENO）以及运动后24小时FENO或组胺支气管高反应性（BHR）的增加之间存在统计学意义的相关性。
通过哮喘中许多重要炎症细胞的影响以及与其他细胞因子的相互作用，支气管上皮释放ET-1可能在哮喘患者运动后支气管收缩后观察到的气道炎症增加中起重要作用。
