Liu J H, Waterhouse A L, Chatterton N J
Department of Chemistry, Tulane University, New Orleans, LA 70118.
Carbohydr Res. 1991 Sep 18;217:43-9. doi: 10.1016/0008-6215(91)84115-u.
The proton and carbon chemical shift assignments of the simplest levan, 6-kestose, [O-beta-D-fructofuranosyl-(2----6)-beta-D-fructofuranosyl-(2 in equilibrium with 1)-alpha-D-glucopyranoside], along with another trisaccharide, neokestose, [O-beta-D-fructofuranosyl-(2----6)-alpha-D-glucopyranosyl-(1 in equilibrium with 2)-beta-D-fructofuranoside], were determined using two-dimensional (2D) homonuclear and heteronuclear n.m.r. methods. The 1H peak of H-1 of the glucose residue was determined by its chemical shift. Using H-H coupling information to this proton, the chemical shifts of most of the proton signals of the glucose moiety were determined. Though the signals from the two fructose residues were very close, a NOESY experiment and long-range C-H correlation experiments allowed their complete carbon and proton assignment. This work completes and corrects literature assignments.
利用二维（2D）同核和异核核磁共振方法，确定了最简单的果聚糖（6-蔗果三糖，[O-β-D-呋喃果糖基-(2→6)-β-D-呋喃果糖基-(2与1平衡)-α-D-吡喃葡萄糖苷]）以及另一种三糖新蔗果三糖（[O-β-D-呋喃果糖基-(2→6)-α-D-吡喃葡萄糖基-(1与2平衡)-β-D-呋喃果糖苷]）的质子和碳化学位移归属。葡萄糖残基H-1的1H峰通过其化学位移确定。利用该质子的H-H耦合信息，确定了葡萄糖部分大多数质子信号的化学位移。尽管两个果糖残基的信号非常接近，但通过NOESY实验和远程C-H相关实验实现了它们完整的碳和质子归属。这项工作完善并修正了文献中的归属。
