重组人白细胞介素-6增强视黄酸对HL-60人髓性白血病细胞的抗增殖和诱导分化作用。

Recombinant human interleukin-6 enhances the antiproliferation and differentiation inducing effects of retinoic acid in HL-60 human myeloid leukaemic cells.

Hassan H T

Institut für Pharmazie, Freie Universität Berlin, Germany.

Clin Exp Pharmacol Physiol. 1991 Dec;18(12):847-51. doi: 10.1111/j.1440-1681.1991.tb01405.x.

Abstract

The effect of recombinant human interleukin-6 on the antiproliferation and differentiation inducing actions of all-trans retinoic acid in HL-60 human myeloid leukaemia cells was studied in short-term liquid suspension culture. 2. Interleukin-6 alone showed no significant effect on HL-60 human myeloid leukaemia cells. 3. The addition of interleukin-6 to all-trans retinoic acid-treated cultures of HL-60 human myeloid leukaemia cells significantly enhanced the desired antiproliferation effect of all-trans retinoic acid. 4. The combination of interleukin-6 with all-trans retinoic acid reduced the doses of all-trans retinoic acid required to induce the same differentiation of HL-60 cells as single agent by between 1.7- and 4.8-fold; that is, the efficacy of all-trans retinoic acid in inducing the differentiation of human myeloid leukaemia HL-60 cells was increased up to 4.8 times by its combination with interleukin-6. 5. The combination of all-trans retinoic acid and interleukin-6 could provide an effective alternative therapy for elderly myeloid leukaemia patients.

摘要

在短期液体悬浮培养中，研究了重组人白细胞介素-6对全反式维甲酸在HL-60人髓性白血病细胞中抗增殖和诱导分化作用的影响。2. 单独的白细胞介素-6对HL-60人髓性白血病细胞无显著影响。3. 在全反式维甲酸处理的HL-60人髓性白血病细胞培养物中添加白细胞介素-6，显著增强了全反式维甲酸所需的抗增殖作用。4. 白细胞介素-6与全反式维甲酸联合使用时，诱导HL-60细胞达到与单一药物相同分化程度所需的全反式维甲酸剂量降低了1.7至4.8倍；也就是说，全反式维甲酸与白细胞介素-6联合使用时，其诱导人髓性白血病HL-60细胞分化的功效提高了4.8倍。5. 全反式维甲酸与白细胞介素-6联合使用可为老年髓性白血病患者提供一种有效的替代疗法。