单粒子电子显微镜中的剂量率效应。
A dose-rate effect in single-particle electron microscopy.
作者信息
Chen James Z, Sachse Carsten, Xu Chen, Mielke Thorsten, Spahn Christian M T, Grigorieff Nikolaus
机构信息
Howard Hughes Medical Institute, Rosenstiel Center-MS029, Brandeis University, 415 South Street, Waltham, MA 02545-9110, USA.
出版信息
J Struct Biol. 2008 Jan;161(1):92-100. doi: 10.1016/j.jsb.2007.09.017. Epub 2007 Oct 1.
Abstract
A low beam intensity, low electron dose imaging method has been developed for single-particle electron cryo-microscopy (cryo-EM). Experiments indicate that the new technique can reduce beam-induced specimen movement and secondary radiolytic effects, such as "bubbling". The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution.
摘要
一种用于单颗粒冷冻电子显微镜（cryo-EM）的低束流强度、低电子剂量成像方法已被开发出来。实验表明，这项新技术可以减少束流诱导的样品移动和二次辐射分解效应，如“起泡”。图像质量的提高，特别是对于多曝光数据采集，将有助于单颗粒冷冻电子显微镜达到更高的分辨率。
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