The Cryo-Electron Microscopy Service Platform, Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Acta Crystallogr D Struct Biol. 2020 Aug 1;76(Pt 8):724-728. doi: 10.1107/S2059798320008347. Epub 2020 Jul 27.
Cryo-electron microscopy (cryo-EM) can be used to elucidate the 3D structure of macromolecular complexes. Driven by technological breakthroughs in electron-microscope and electron-detector development, coupled with improved image-processing procedures, it is now possible to reach high resolution both in single-particle analysis and in cryo-electron tomography and subtomogram-averaging approaches. As a consequence, the way in which cryo-EM data are collected has changed and new challenges have arisen in terms of microscope alignment, aberration correction and imaging parameters. This review describes how high-end data collection is performed at the EMBL Heidelberg cryo-EM platform, presenting recent microscope implementations that allow an increase in throughput while maintaining aberration-free imaging and the optimization of acquisition parameters to collect high-resolution data.
低温电子显微镜（cryo-EM）可用于阐明大分子复合物的 3D 结构。受电子显微镜和电子探测器发展方面的技术突破的推动，再加上改进的图像处理程序，现在在单颗粒分析以及低温电子断层扫描和亚断层平均方法中都有可能达到高分辨率。因此，低温电子显微镜数据的采集方式发生了变化，在显微镜对准、像差校正和成像参数方面出现了新的挑战。这篇综述描述了如何在 EMBL 海德堡低温电子显微镜平台上进行高端数据采集，介绍了最近的显微镜实现方法，这些方法可以在保持无像差成像的同时提高通量，并优化采集参数以收集高分辨率数据。
