Wang Wei-Dong, Huang Yu-Ming, Shu Wei-Qun, Cao Jia
College of Chemistry and Chemical Engineering, Southwest University, The Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China.
J Chromatogr A. 2007 Nov 30;1173(1-2):27-36. doi: 10.1016/j.chroma.2007.10.027. Epub 2007 Oct 16.
Multiwalled carbon nanotubes (MWCNTs) were used as a novel kind of solid-phase extraction adsorbents in this work as well as an analytical method based on MWCNTs solid-phase extraction (SPE) combined with high-performance liquid chromatography (HPLC) was established for the determination of polycyclic aromatic hydrocarbons (PAHs), some of which belong to typical persistent organic pollutants (POPs) owing to their carcinogenicity and endocrine disrupting activity. Several conditions that probably affected the extraction efficiency including the eluent volume, sample flow rate, sample pH and the sample volume were optimized in detail. The characteristic data of analytical performance were determined to investigate the sensitivity and precision of the method, and the method was applied to the determination of PAHs in environmental water samples such as river water sample, tap water sample and wastewater sample from the constructed wetland effluent. The experimental results indicated that there were excellent linear relationship between peak area and the concentration of PAHs over the range of 0.04-100 microg L(-1), and the precisions (RSD) were 1.7-4.8% under the optimal conditions. The detection limits of proposed method for the studied PAHs were 0.005-0.058 microg L(-1) (S/N=3). The recoveries of PAHs spiked in environmental water samples ranged from 78.7 to 118.1%. It was concluded that MWCNTs packed cartridge coupled with HPLC was an excellent alternative for the routine analysis of PAHs at trace level.
在本研究中，多壁碳纳米管（MWCNTs）被用作一种新型的固相萃取吸附剂，并建立了一种基于MWCNTs固相萃取（SPE）结合高效液相色谱（HPLC）的分析方法，用于测定多环芳烃（PAHs），其中一些PAHs因其致癌性和内分泌干扰活性而属于典型的持久性有机污染物（POPs）。详细优化了几个可能影响萃取效率的条件，包括洗脱液体积、样品流速、样品pH值和样品体积。测定了分析性能的特征数据，以研究该方法的灵敏度和精密度，并将该方法应用于环境水样中PAHs的测定，如河水样、自来水样和人工湿地出水的废水样。实验结果表明，在0.04 - 100 μg L⁻¹范围内，PAHs的峰面积与浓度之间存在良好的线性关系，在最佳条件下精密度（RSD）为1.7 - 4.8%。该方法对所研究的PAHs的检测限为0.005 - 0.058 μg L⁻¹（S/N = 3）。加标环境水样中PAHs的回收率在78.7%至118.1%之间。结果表明，MWCNTs填充柱结合HPLC是痕量水平PAHs常规分析的一种优秀替代方法。
