Contreras-Solis I, Gomez-Brunet A, Encinas T, Gonzalez-Bulnes A, Santiago-Moreno J, Lopez-Sebastian A
Departamento de Reproducción Animal, INIA, Av. Puerta de Hierro, s/n, Madrid 28040, Spain.
Res Vet Sci. 2008 Aug;85(1):162-5. doi: 10.1016/j.rvsc.2007.09.011. Epub 2007 Oct 30.
This study aimed to determine the best vehicle and administration route for progesterone administration in sheep. In a first replicate, single intramuscular doses of 25mg progesterone were administered to ewes previously ovariectomized, either in propylene glycol (group IM-PG, n=6) or olive oil (group IM-OO, n=5). In a second replicate, the same solutions of progesterone were administered subcutaneously to the same ewes (groups SC-PG, n=6, and SC-OO, n=5). In the present study, the best pharmacokinetic results of a single dose of 25mg of progesterone were obtained, both using PG and OO as vehicles, by the subcutaneous route. Thus, progesterone remained in plasma for a longer time after subcutaneous administration in PG than in OO (t(1/2beta): 60.65+/-13.07 vs. 27.51+/-3.59 h; P<0.05); the mean residence time being higher in SC-PG than in SC-OO group (88.99+/-18.36 vs. 41.04+/-5.31h; P<0.05). However, both vehicles allowed maintained plasma levels 0.5 ng/ml for at least 30 h, so any of these treatments may be efficiently used for administration of exogenous progesterone.
本研究旨在确定绵羊体内孕酮给药的最佳载体和给药途径。在首次重复实验中，对先前已切除卵巢的母羊单次肌内注射25mg孕酮，分别使用丙二醇（肌内注射-丙二醇组，n = 6）或橄榄油（肌内注射-橄榄油组，n = 5）作为载体。在第二次重复实验中，将相同的孕酮溶液皮下注射给相同的母羊（皮下注射-丙二醇组，n = 6，皮下注射-橄榄油组，n = 5）。在本研究中，以丙二醇和橄榄油作为载体，通过皮下途径单次注射25mg孕酮均获得了最佳药代动力学结果。因此，皮下注射后，孕酮在血浆中的停留时间在丙二醇组比在橄榄油组更长（β半衰期：60.65±13.07 vs. 27.51±3.59小时；P<0.05）；皮下注射-丙二醇组的平均驻留时间高于皮下注射-橄榄油组（88.99±18.36 vs. 41.04±5.31小时；P<0.05）。然而，两种载体都能使血浆水平维持在0.5 ng/ml至少30小时，因此这些处理中的任何一种都可有效地用于外源性孕酮的给药。
