北里研究所生产的无细胞百日咳疫苗的比较生物学活性。

Comparative biological activities of acellular pertussis vaccines produced by Kitasato.

作者信息

Watanabe M, Izumiya K, Sato T, Yoshino K, Nakagawa N, Ohoishi M, Hoshino M

机构信息

Division of Bacterial Products, Kitasato Institute, Tokyo, Japan.

出版信息

Kitasato Arch Exp Med. 1991 Apr;64(1):31-42.

PMID:1798236

Abstract

The quality of 14 lots of acellular pertussis-diphtheria-tetanus (AC-PDT) vaccines manufactured by the Kitasato Institute during the period 1987-1990 were investigated. The geometric means of HSU, LPU, and BWDU were 0.078, 0.257, and 7.33 per ml respectively. The potency was higher than 14 IU per ml. These results indicated the consistency of the Kitasato AC-PDT vaccines. The antibody response to the AC-PDT vaccines was measured in primary and secondary vaccinated mice by ELISA. IgG antibody response to FHA and PT was obtained in all immunized mice (P less than 0.001) after the primary injection. In contrast, IgG antibody response to fimbriae 2 showed a significant titer rise (P less than 0.001) after the booster injection. The results indicated that the Kitasato AC-P vaccines consisted of protein, PT and FHA as the major antigens, and a little agglutinogen as the minor antigen.

摘要

对北里研究所1987年至1990年期间生产的14批无细胞百日咳-白喉-破伤风（AC-PDT）疫苗的质量进行了调查。每毫升的血细胞凝集单位（HSU）、致死保护单位（LPU）和体重增加单位（BWDU）的几何平均值分别为0.078、0.257和7.33。效力高于每毫升14国际单位（IU）。这些结果表明北里AC-PDT疫苗具有一致性。通过酶联免疫吸附测定（ELISA）法在初次和二次接种疫苗的小鼠中测量了对AC-PDT疫苗的抗体反应。初次注射后，所有免疫小鼠均获得了针对丝状血凝素（FHA）和百日咳毒素（PT）的IgG抗体反应（P<0.001）。相比之下，二次注射后，针对2型菌毛的IgG抗体反应出现了显著的滴度升高（P<0.001）。结果表明，北里AC-P疫苗以蛋白质、PT和FHA作为主要抗原，以少量凝集原为次要抗原。