Perju Andreia Claudia, Mândrescu Mariana, Spac A F, Dorneanu V
Facultatea de Farmacie, Disciplina de Chimie analitică, Universitatea de Medicină i Farmacie "Gr.T. Popa" Iaşi.
Rev Med Chir Soc Med Nat Iasi. 2007 Apr-Jun;111(2):535-9.
Nimesulide is a nonsteroidal antiinflammatory (NSAID) drug whose mechanism of action is characterized by selective inhibition of cyclooxygenase-2. Nimesulide have analgesic and antipyretic properties.
For nimesulide analysis we developed a spectrophotometric method based on reduction of the nitro group of nimesulide by zinc and hydrochloric acid followed by diazotization and coupling with thymol (5-methyl-2-(1-methylethyl) phenol) in ammonia medium to form a stable chromophore which absorbs at 476 nm. There were established the practical working conditions. The developed method was validated.
The method showed a good linearity in the range of 5 - 40 microg / sample (the correlation coefficient r = 0.9990). The detection limit (LD) was 0.99 microg/sample and the quantification limit (LQ) was 3.32 microg/sample. There were established the system precision (RSD = 1.70%), the precision (RSD < 2.2%) and the accuracy (recovery in the range 97.66 - 102.69% with a mean recovery of 99.81%) of the method.
A new method for nimesulide spectrophotometric quantification in visible has been developed.
