Maier S S, Maier Vasilica, Bucişcanu Ingrid, Pruneanu Melinda
Facultatea de Textile - Pielărie, Catedra de Technologia chimică a produselor din piele, Universitatea Tehnică "Gh. Asachi" Iaşi.
Rev Med Chir Soc Med Nat Iasi. 2007 Apr-Jun;111(2):544-8.
Biomedical applications of collagen are conditioned by its use in a state very close to the native state, as it concerns conformation and reactivity. Minimal denaturation implies that both the collagen structure and the side amino acid groups are kept intact. The bulk of alterations take place in phases preceding the extraction itself.
The authors have established an optimal protocol for preprocessing of young bovine hides, i.e. to obtain a maximum solubilisation yield when a standard proteases-based solubilisation technique was applied.
The sequence: unhairing--loosing--cleaning--labilisation of corium tissue was taken in account. The effect of the treating composition upon the deamination degree of the extracted collagen was determined through a binary gradient HPLC technique, on a ProPac WCX-10 analytical column. The optimal range of loosing--cleaning recipe components was found to be between 18 ... 27% dithiotreitol (DDT), 12 ... 20% N-ethyl-acetamide (NEM) and 30 ... 53% iodide-acetamide (IDAM).
胶原蛋白的生物医学应用取决于其在非常接近天然状态下的使用，因为这涉及到构象和反应性。最小程度的变性意味着胶原蛋白结构和侧链氨基酸基团都保持完整。大部分改变发生在提取本身之前的阶段。
作者建立了一种用于幼年牛皮预处理的最佳方案，即当应用基于标准蛋白酶的溶解技术时，获得最大的溶解产率。
考虑了脱毛 - 松弛 - 清洁 - 真皮组织不稳定的顺序。通过二元梯度高效液相色谱技术，在ProPac WCX - 10分析柱上测定处理组合物对提取的胶原蛋白脱氨基程度的影响。发现松弛 - 清洁配方成分的最佳范围为18……27%二硫苏糖醇（DDT）、12……20% N - 乙基乙酰胺（NEM）和30……53%碘乙酰胺（IDAM）。
