Manivannan P, Jaleel C Abdul, Sankar B, Kishorekumar A, Murali P V, Somasundaram R, Panneerselvam R
Stress Physiology Lab, Department of Botany, Annamalai University, Annamalainagar, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2008 Mar 15;62(1):58-63. doi: 10.1016/j.colsurfb.2007.09.019. Epub 2007 Sep 21.
Experiments were conducted to study the effects of different sodium salts viz., sodium chloride (NaCl), sodium sulphate (Na(2)SO(4)) and sodium carbonate (Na(2)CO(3)) on growth, dry matter production, mineral contents, biochemical constituent and enzyme activities of sunflower (Helianthus annuus L.). The germinating sunflower seeds were treated with 10, 20 and 50mM NaCl and Na(2)SO(4) and 5, 10 and 15 mM Na(2)CO(3). The seedling growth, minerals, chlorophyll content and biochemicals like protein and free amino acid contents with enzyme activities like ATPase and protease were analysed on 8 DAS. The seedlings were separated into root, stem, leaf and cotyledon on 8 DAS. All the treatments decreased the germination percentage; shoot length, root length, leaf area and dry weight, chlorophyll and protein contents significantly. Potassium, sodium and free amino acid contents; activities of ATPase and protease were increased when compared to control. This effect was very high in the Na(2)CO(3) treated seedlings this was followed by Na(2)SO(4) and NaCl treated seedlings. From the results of this investigation, it is clear that, the sunflower seedlings were affected significantly in the Na(2)CO(3) treatments, and followed by Na(2)SO(4) and NaCl treatments.
进行了实验以研究不同钠盐，即氯化钠（NaCl）、硫酸钠（Na₂SO₄）和碳酸钠（Na₂CO₃）对向日葵（Helianthus annuus L.）生长、干物质产量、矿物质含量、生化成分和酶活性的影响。将发芽的向日葵种子用10、20和50mM的NaCl和Na₂SO₄以及5、10和15mM的Na₂CO₃处理。在播种后8天（DAS）分析幼苗生长、矿物质、叶绿素含量以及蛋白质和游离氨基酸含量等生化成分以及ATP酶和蛋白酶等酶活性。在播种后8天，将幼苗分为根、茎、叶和子叶。所有处理均显著降低了发芽率、苗长、根长、叶面积和干重、叶绿素和蛋白质含量。与对照相比，钾、钠和游离氨基酸含量以及ATP酶和蛋白酶的活性增加。这种影响在Na₂CO₃处理的幼苗中非常高，其次是Na₂SO₄和NaCl处理的幼苗。从这项研究的结果可以清楚地看出，向日葵幼苗在Na₂CO₃处理中受到的影响最大，其次是Na₂SO₄和NaCl处理。
