低氧诱导因子-1α介导的小鼠软骨细胞中易化葡萄糖转运蛋白-1和葡萄糖转运蛋白-3的低氧调节
Hypoxia regulation of facilitated glucose transporter-1 and glucose transporter-3 in mouse chondrocytes mediated by HIF-1alpha.
作者信息
Ren Bu-Fang, Deng Lian-Fu, Wang Jun, Zhu Ya-Ping, Wei Li, Zhou Qi
机构信息
Shanghai Institute of Traumatology & Orthopaedics and Department of Orthopaedics, Ruijin Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200025, China.
出版信息
Joint Bone Spine. 2008 Mar;75(2):176-81. doi: 10.1016/j.jbspin.2007.05.012. Epub 2007 Aug 31.
OBJECTIVES
Chondrocytes reside in a hypoxic environment; they utilize glucose as the main energy source. Facilitated glucose transport represents the first rate-limiting step in glucose metabolism.
RESULTS
Here we demonstrated that hypoxia induced an elevated expression level of GLUT-1 and -3 in chondrocytes. Also elevated was hypoxia inducible factor-1alpha (HIF-1alpha) protein level. On the contrary, deletion of HIF-1alpha led to complete loss of the hypoxia-induced gene expression of GLUT-1 and 3.
CONCLUSION
These results suggested that chondrocytes had the capacity to detect and respond to low oxygen availability with changes in expression of oxygen-regulated genes, and that hypoxia regulation of facilitated GLUT-1 and -3 was mediated by HIF-1alpha.
目的
软骨细胞处于低氧环境中；它们利用葡萄糖作为主要能量来源。易化葡萄糖转运是葡萄糖代谢的首个限速步骤。
结果
我们在此证明，低氧诱导软骨细胞中GLUT-1和GLUT-3的表达水平升高。低氧诱导因子-1α（HIF-1α）蛋白水平也升高。相反，HIF-1α的缺失导致低氧诱导的GLUT-1和GLUT-3基因表达完全丧失。
结论
这些结果表明，软骨细胞有能力通过氧调节基因表达的变化来检测并响应低氧环境，且低氧对易化型GLUT-1和GLUT-3的调节是由HIF-1α介导的。
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