Cai Wei-Wen
Department of Molecular and Human Genetics, Houston, TX, USA.
Methods Mol Biol. 2007;381:105-19. doi: 10.1007/978-1-59745-303-5_5.
Array-based comparative genomic hybridization (array CGH) is becoming a prominent genomic technology with many important applications in biomedical research. Although several platforms of this technology have been published, successful implementation of this technology still demands technical expertise. Here, we describe the technology that has been developed and improved in the past few years are described. Our array CGH technology is primarily based on robust and readily implemented array production method. We also developed related protocols for using our bacterial artifical chromosomes CGH microarrays. This technology was successfully used to detect DNA copy-number alterations in various mouse and human samples.
基于芯片的比较基因组杂交技术（芯片比较基因组杂交，array CGH）正成为一项重要的基因组技术，在生物医学研究中有许多重要应用。尽管该技术的多个平台已发表，但要成功实施这项技术仍需要专业技术知识。在此，我们描述了过去几年中开发和改进的技术。我们的芯片比较基因组杂交技术主要基于稳健且易于实施的芯片生产方法。我们还开发了使用细菌人工染色体芯片比较基因组杂交微阵列的相关方案。这项技术已成功用于检测各种小鼠和人类样本中的DNA拷贝数变化。
