Miyoshi Kazuchika, Fujimoto Yumi, Mori Hironori, Yoshida Mitsutoshi
Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
J Reprod Dev. 2008 Feb;54(1):42-5. doi: 10.1262/jrd.19034. Epub 2007 Nov 2.
The present study was carried out to examine the activation and parthenogenetic development of pig oocytes after exposure to ultrasound in sorbitol media supplemented with different concentrations of Ca2+. The activation rates (68.8-75.6%) of oocytes exposed to ultrasound in media containing 0.1-1.0 mM Ca2+ were significantly (P<0.05) higher than those (54.3-58.3%) of oocytes exposed to ultrasound in media containing 0-0.05 mM Ca2+. When oocytes were exposed to ultrasound in media containing 0.1-0.5 mM Ca2+, the blastocyst formation rates (20.5-21.3%) were significantly (P<0.05) higher than those (3.3-6.0%) of oocytes exposed to ultrasound in media containing 0, 0.05 or 1.0 mM Ca2+. The results of the present study showed that the concentration of Ca2+ in the medium used for exposure to ultrasound affects the activation and parthenogenetic development of pig oocytes and showed that the optimal Ca2+ concentration is 0.1-0.5 mM.
本研究旨在检测猪卵母细胞在补充不同浓度Ca2+的山梨醇培养基中经超声处理后的激活及孤雌发育情况。在含有0.1 - 1.0 mM Ca2+的培养基中经超声处理的卵母细胞激活率（68.8 - 75.6%）显著（P<0.05）高于在含有0 - 0.05 mM Ca2+的培养基中经超声处理的卵母细胞激活率（54.3 - 58.3%）。当卵母细胞在含有0.1 - 0.5 mM Ca2+的培养基中经超声处理时，囊胚形成率（20.5 - 21.3%）显著（P<0.05）高于在含有0、0.05或1.0 mM Ca2+的培养基中经超声处理的卵母细胞囊胚形成率（3.3 - 6.0%）。本研究结果表明，超声处理所用培养基中的Ca2+浓度会影响猪卵母细胞的激活及孤雌发育，并表明最佳Ca2+浓度为0.1 - 0.5 mM。
