Iqbal A, Khan A S, Khan I A, Awan F S, Ahmad A, Khan A A
Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan.
Genet Mol Res. 2007 Aug 7;6(3):476-81.
The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li's similarity matrix ranged from 86.2 to 93.0%, which indicated a narrow genetic base among the genotypes. The maximum similarity, 93.0%, was observed between 12WLRG/1-12 and WL-43. The local variety, Chenab-70, showed the lowest similarity with the exotic types. We conclude that random amplified polymorphic DNA analysis can be used for the characterization and grouping of wheat genotypes; these results will be helpful in our wheat breeding program.
通过随机扩增多态性DNA方法，对7个小麦基因型、6个外来基因型和1个当地品种的遗传分化程度进行了估计。15个随机引物共产生了112个DNA片段，每个引物平均约有7.4条带。在这112个片段中，有50个片段在7个小麦基因型间表现出多态性。Nei和Li的相似性矩阵范围为86.2%至93.0%，这表明这些基因型间的遗传基础较窄。在12WLRG/1-12和WL-43之间观察到最大相似性，为93.0%。当地品种Chenab-70与外来类型的相似性最低。我们得出结论，随机扩增多态性DNA分析可用于小麦基因型的鉴定和分组；这些结果将有助于我们的小麦育种计划。
