Jakab E, Schmidt T, Hernádi F
Department of Chemotherapeutical Research, BIOGAL Pharmeceutical Works, Debrecen, Hungary.
Prep Biochem. 1991;21(2-3):105-23. doi: 10.1080/10826069108018007.
DNA polymerases of Candida albicans were purified to near homogeneity. Three well distinguished peaks of DNA polymerase activity (Enzyme I, II and III respectively) were obtained by DEAE-Sephacel chromatography. This purification step was followed by column chromatographies on Sepharose 6B and denatured DNA-cellulose. The enzymes' molecular mass and biochemical properties, including their inhibition by aphidicolin, were studied. Molecular mass was determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and was found to be 110 kDa for Enzyme I, 80 kDa for Enzyme II and 50 kDa for Enzyme III.
白色念珠菌的DNA聚合酶被纯化至近乎同质。通过DEAE-琼脂糖凝胶柱层析获得了三个明显区分的DNA聚合酶活性峰（分别为酶I、II和III）。该纯化步骤之后是在琼脂糖凝胶6B和变性DNA纤维素上进行柱层析。研究了这些酶的分子量和生化特性，包括它们对阿非迪霉素的抑制作用。分子量通过在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳测定，发现酶I为110 kDa，酶II为80 kDa，酶III为50 kDa。
