Bera Manav B, Panesar Parmjit S, Panesar Reeba, Singh Bahadur
Biotechnology Research Laboratory, Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal, 148106, Punjab, India.
Bioprocess Biosyst Eng. 2008 Jun;31(4):379-84. doi: 10.1007/s00449-007-0172-6. Epub 2007 Nov 7.
The effect of different process variables of reverse micelle extraction process like pH, addition of surfactant (AOT) concentration and potassium chloride (KCl) concentration on amylase recovery has been studied and analysed. Solid-state fermentation was used for the production of amylase enzyme. Response surface methodology (RSM) using central composite rotatable design (CCRD) was employed to analyse and optimize the enzyme extraction process. The regression analysis indicates that the effect of AOT concentration, and KCl concentration were significant, whereas the effect of pH was non-significant on enzyme recovery. For the maximum recovery of enzyme, the optimum operating condition for pH, AOT concentration (M) and KCl concentration were 10.43, 0.05 and 1.00, respectively. Under these optimal conditions, the enzyme recovery was 83.16%.
研究并分析了反胶束萃取过程中不同工艺变量（如pH值、表面活性剂（AOT）添加浓度和氯化钾（KCl）浓度）对淀粉酶回收率的影响。采用固态发酵法生产淀粉酶。运用中心复合旋转设计（CCRD）的响应面法（RSM）对酶提取过程进行分析和优化。回归分析表明，AOT浓度和KCl浓度对酶回收率的影响显著，而pH值的影响不显著。为实现酶的最大回收率，pH值、AOT浓度（M）和KCl浓度的最佳操作条件分别为10.43、0.05和1.00。在这些最佳条件下，酶回收率为83.16%。
