Jiang Tao, Liang Zhong, Chen Juan, He Ji-Jun, Lu Lü, Ma Wei-Min, Liu Zai-Xin, Liu Xiang-Tao
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agrcultrual Sciences, Lanzhou 730046, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Nov;23(11):1021-4.
To establish a colloid gold-immunochromatography assay (GICA) for detecting type Asia I foot-and-mouth disease virus (FMDV).
Colloidal gold was obtained by reducing the gold chloride with sodium citrate and then it was coupled with the purified anti-FMDV type Asia I antibody.The purified anti-FMDV type Asia I antibody and the goat anti-Guinea pig IgG were wrapped onto the nitrocellulose membrane as test line (T line) and control line (C line). The GICA strip was assembled with the purified antibody labelled with colloidal gold and the nitrocellulose containing antibody. The sensitivity, specificity and stability of GICA strip were evaluated in the diagnosis of FMD viral antigen from clinical samples.
The strip was highly sensitive to FMDV type Asia I(0.116 mg/L) and it had the same result for positive specimens tested thrice. Cross tests proved no cross reaction was found with other serotype FMDV and Swine vesicular disease (SVD) antigen.The corresponding rate of GICA with RIHA was 96.87% for detecting the field sample. The stably test found the strip could be stored for 12 months.
The GICA strip is a simple and rapid immunochromatographic test strip for the detection of FMDV type Asia I with high sensitivity and specificity.
建立一种检测亚洲I型口蹄疫病毒(FMDV)的胶体金免疫层析试验(GICA)。
用柠檬酸钠还原氯金酸制得胶体金,然后将其与纯化的抗亚洲I型FMDV抗体偶联。将纯化的抗亚洲I型FMDV抗体和山羊抗豚鼠IgG包被在硝酸纤维素膜上作为检测线(T线)和对照线(C线)。用胶体金标记的纯化抗体和含抗体的硝酸纤维素膜组装成GICA试纸条。对临床样本中的FMD病毒抗原诊断,评估GICA试纸条的敏感性、特异性和稳定性。
该试纸条对亚洲I型FMDV高度敏感(0.116 mg/L),对阳性标本进行三次检测结果相同。交叉试验证明与其他血清型FMDV和猪水泡病(SVD)抗原无交叉反应。检测现场样本时,GICA与反向间接血凝试验(RIHA)的符合率为96.87%。稳定性试验发现该试纸条可保存12个月。
GICA试纸条是一种简单快速的免疫层析试纸条,用于检测亚洲I型FMDV,具有高敏感性和特异性。