Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.
J Virol Methods. 2010 Feb;163(2):474-6. doi: 10.1016/j.jviromet.2009.09.022. Epub 2009 Oct 6.
A lateral flow device (LFD) for the detection of foot-and-mouth disease virus (FMDV) of the SAT 2 serotype was developed using a monoclonal antibody (Mab 2H6). The performance of the LFD was evaluated in the laboratory on suspensions of vesicular epithelia: 305 positive for FMDV type SAT 2 from suspected cases of vesicular disease collected from 30 countries and 1002 samples shown to be negative for FMDV type SAT 2 collected from 67 countries between 1968 and 2008. The diagnostic sensitivity of the LFD for FMDV type SAT 2 was higher at 88% compared to 79% obtained by the reference method of antigen ELISA, and the diagnostic specificity of the LFD was approximately 99% compared to 100% for the ELISA. The device recognized FMDV strains of wide diversity within the FMDV SAT 2 serotype and gave a superior performance for their detection compared to the 1F10 LFD which had been developed previously and shown to perform less well for the detection of FMDVs of this particular serotype. Reactions in the SAT 2 2H6 LFD with the viruses of other FMDV serotypes and swine vesicular disease (which produces a clinically indistinguishable syndrome in pigs), did not occur. These data illustrate the potential for the LFD to be employed to complement the 1F10 device next to the animal in the pen-side diagnosis of FMD, for providing rapid and objective support to veterinarians in their clinical judgment of the disease and for specific confirmation of a FMDV type SAT 2 infection.
一种用于检测口蹄疫病毒（FMDV）SAT 2 血清型的侧向流动装置（LFD）是使用单克隆抗体（Mab 2H6）开发的。该 LFD 的性能在实验室中对水疱上皮的悬浮液进行了评估：来自 30 个国家疑似水疱病的 305 个 SAT 2 型 FMDV 阳性样本，以及来自 1968 年至 2008 年的 67 个国家的 1002 个 SAT 2 型 FMDV 阴性样本。与抗原 ELISA 参考方法相比，LFD 对 SAT 2 型 FMDV 的诊断敏感性为 88%，高于 79%，而 LFD 的诊断特异性与 ELISA 相比约为 99%。该装置识别 SAT 2 血清型内 FMDV 株的多样性，并在检测 FMDV 方面表现优于先前开发的 1F10 LFD，后者在检测该特定血清型的 FMDV 时性能较差。与其他 FMDV 血清型和猪水疱病（在猪中产生临床上无法区分的综合征）的病毒在 SAT 2 2H6 LFD 中的反应并未发生。这些数据表明，LFD 有可能在动物围栏旁的口蹄疫诊断中与 1F10 设备一起使用，为兽医在疾病的临床判断中提供快速和客观的支持，并对口蹄疫病毒 SAT 2 感染进行特定确认。
