微乳液电动色谱法用于牛蒡子及其制剂中牛蒡苷和牛蒡子苷元的分离

Microemulsion electrokinetic chromatography for the separation of arctiin and arctigenin in Fructus Arctii and its herbal preparations.

作者信息

Lü Wenjuan, Chen Yonglei, Zhang Yuxia, Ding Xiuping, Chen Hongli, Liu Mancang

机构信息

Department of Chemistry, Lanzhou University, Lanzhou 730000, China.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Dec 1;860(1):127-33. doi: 10.1016/j.jchromb.2007.10.017. Epub 2007 Oct 22.

DOI:10.1016/j.jchromb.2007.10.017

PMID:17988959

Abstract

A microemulsion electrokinetic chromatography method was used to separate arctiin and arctigenin in Fructus Arctii and its herbal preparations. The separation of arctiin and arctigenin was performed using a 1-butanol-SDS-ethyl acetate-water microemulsion in 10mM sodium tetraborate buffer. The analytes were baseline-resolved within 4 min. In the concentration range 5-500 microg/mL, the calibration curves reveal linear relationships between the peak area for each analyte and its concentration (correlation coefficients: 0.9993 for arctiin and 0.9998 for arctigenin). The method was applied to the analysis of arctiin and arctigenin in herbal preparations, and the recoveries were 98.7-103.1% for arctiin and 97.6-103.2% for arctigenin, respectively.

摘要

采用微乳液电动色谱法分离牛蒡子及其制剂中的牛蒡苷和牛蒡子苷元。使用含1-丁醇-十二烷基硫酸钠-乙酸乙酯-水的微乳液，在10mM硼酸钠缓冲液中对牛蒡苷和牛蒡子苷元进行分离。分析物在4分钟内实现基线分离。在5-500μg/mL浓度范围内，校准曲线显示各分析物的峰面积与其浓度之间呈线性关系（相关系数：牛蒡苷为0.9993，牛蒡子苷元为0.9998）。该方法应用于制剂中牛蒡苷和牛蒡子苷元的分析，牛蒡苷的回收率为98.7-103.1%，牛蒡子苷元的回收率为97.6-103.2%。