Sitohy Mahmoud, Billaudel Sylviane, Haertlé Thomas, Chobert Jean-Marc
UR 1268 Biopolymères Interactions Assemblages, Institut National de la Recherche Agronomique, 44300 Nantes, France.
J Agric Food Chem. 2007 Dec 12;55(25):10214-20. doi: 10.1021/jf0724421. Epub 2007 Nov 9.
The antiviral activity of methylated alpha-lactalbumin (Met-ALA), methylated and ethylated beta-lactoglobulins (Met- and Et-BLG) was evaluated against acyclovir (ACV)-sensitive and -resistant strains of herpes simplex virus type 1 (HSV-1) and compared to that of ACV and L-polylysines (4-15 kDa) using fixed or suspended Vero cell lines. Esterified whey proteins and their peptic hydrolyzates displayed protective action against HSV-1, which was relatively lower than that induced by ACV or L-polylysines. The higher activity of L-polylysines was maintained against an ACV-resistant strain of HSV-1, whereas ACV lost much of its activity. The mean 50% inhibitory concentration (IC50) was about 0.8-0.9 microg/mL for L-polylysines against ACV-sensitive and -resistant strains of HSV-1 when using two concentrations of virus (50% and 100% cytopathic effect, CPE). The IC50 values of ACV against the sensitive strain of HSV-1 were 3 and 15 microg/mL when using the low and high concentrations of virus, respectively. When using 50% CPE, IC50 values for esterified whey proteins ranged from 20 to 95 microg/mL, depending on the nature of the ester group, the degree of esterification, and the nature of the protein. Using the real-time PCR technique, it was shown that Met-ALA inhibited HSV-1 replication.
评估了甲基化α-乳白蛋白（Met-ALA）、甲基化和乙基化β-乳球蛋白（Met-和Et-BLG）对1型单纯疱疹病毒（HSV-1）阿昔洛韦（ACV）敏感和耐药菌株的抗病毒活性，并使用固定或悬浮的Vero细胞系将其与ACV和L-聚赖氨酸（4-15 kDa）的活性进行比较。酯化乳清蛋白及其胃蛋白酶水解产物对HSV-1具有保护作用，但其保护作用相对低于ACV或L-聚赖氨酸诱导的保护作用。L-聚赖氨酸对HSV-1的ACV耐药菌株仍保持较高活性，而ACV则失去了大部分活性。当使用两种病毒浓度（50%和100%细胞病变效应，CPE）时，L-聚赖氨酸对HSV-1的ACV敏感和耐药菌株的平均50%抑制浓度（IC50）约为0.8-0.9μg/mL。当使用低浓度和高浓度病毒时，ACV对HSV-1敏感菌株的IC50值分别为3和15μg/mL。当使用50% CPE时，酯化乳清蛋白的IC50值范围为20至95μg/mL，具体取决于酯基的性质、酯化程度和蛋白质的性质。使用实时PCR技术表明，Met-ALA抑制HSV-1复制。
