Provenzano G, Marino L, Craxì A
Clínica Médica R, Istituto di Medicina Generale e Pneumologia, Ospedale V. Cervello, Università di Palermo, Italy.
Allergol Immunopathol (Madr). 1991 May-Jun;19(3):119-22.
The presence of autoantibodies in 125 patients with cryptogenic chronic hepatitis (CH) was assessed by indirect immunofluorescence (IFL) on standard tissue sections and on Hep-2 cells. Smooth muscle antibodies (SMA) were found in a high percentage of patients without any difference between the anti-HBc positive and the anti-HBc negative subsets, but the antiactin pattern, suggestive of autoimmunity, was detected only in a small number of cases. Low-titer antinuclear antibodies (ANA) were found in CH but also in healthy controls on Hep-2 cells. No significant differences were found in the prevalence of SMA and ANA positivity in various histologic subgroups of CH. Amongst 37 patients with primary biliary cirrhosis (PBC) the use of Hep-2 cells allowed better evaluation of ANA patterns and identified the multiple nuclear dots (MND) pattern, unrecognisable on tissue sections, in 9 cases.
通过对标准组织切片和Hep - 2细胞进行间接免疫荧光（IFL）检测，评估了125例隐源性慢性肝炎（CH）患者体内自身抗体的存在情况。在高比例患者中发现了平滑肌抗体（SMA），抗 - HBc阳性和抗 - HBc阴性亚组之间无差异，但仅在少数病例中检测到提示自身免疫的抗肌动蛋白模式。在CH患者中发现了低滴度抗核抗体（ANA），但在Hep - 2细胞检测的健康对照中也有发现。CH的不同组织学亚组中SMA和ANA阳性率无显著差异。在37例原发性胆汁性肝硬化（PBC）患者中，使用Hep - 2细胞能更好地评估ANA模式，并在9例患者中识别出在组织切片上无法识别的多核点（MND）模式。
