van Noort J M, van den Berg P, Mattern I E
TNO-Medical Biological Laboratory, Rijswijk, The Netherlands.
Anal Biochem. 1991 Nov 1;198(2):385-90. doi: 10.1016/0003-2697(91)90444-x.
A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.
本文描述了一种用于可视化复杂生物样品中单个蛋白酶的方法。在单个色谱步骤中，蛋白酶可通过与固定化杆菌肽（一种肽类抗生素）选择性结合而与其他生物分子分离。解吸后，蛋白酶可通过SDS-聚丙烯酰胺凝胶电泳进行分离。该方法的应用体现在黑曲霉分泌的蛋白酶的可视化中。
