Cressoni Elthon Silveira, Avanci Luiz Ernesto, Braile Domingo Marcolino, Lima-Oliveira Ana Paula Marques, Taboga Sebastião Roberto, Martins Antonio Sérgio, Oyama Rosa Sayoko Kawasaki, Oliveira Marcos Aurélio Barbosa de
Medical School of the Paulista StateUniversity, Botucatu, SP.
Rev Bras Cir Cardiovasc. 2007 Jan-Mar;22(1):24-32. doi: 10.1590/s0102-76382007000100008.
To experimentally compare the structural and ultrastructural changes in isolated hypertrophied rabbits' hearts submitted to cardiac arrest protected using sanguineous and crystalloid cardioplegia solutions.
The study comprised two experimental groups and one control group. In Experimental Group I, cardiac arrest was achieved by the continuous infusion of tepid sanguineous cardioplegia solution. In Experimental Group II, cardiac arrest was obtained by an intermittent infusion of a cold crystalloid cardioplegia solution. In the Control Group the hearts were submitted to normothermic anoxic arrest for 45 minutes. After the procedures, eight samples of the left ventricle lateral wall were collected and fixed in 10% formaldehyde and 2.5% glutaraldehyde for structural and ultrastructural analysis.
The structural and ultrastructural results demonstrated that the hearts submitted to cardiac arrest protected by continuous tepid sanguineous cardioplegia, Group I, were better preserved and with less accentuated cellular alterations compared to those submitted to cardiac arrest protected using intermittent cold crystalloid cardioplegia and the Control Group.
Continuous tepid sanguineous cardioplegia was more efficient in the preservation of the structural and ultrastructural integrity of the myocardium when compared to intermittent cold crystalloid cardioplegia.
通过实验比较使用含血停搏液和晶体停搏液保护的离体肥厚兔心脏在心脏骤停后的结构和超微结构变化。
本研究包括两个实验组和一个对照组。在实验组I中，通过持续输注温热含血停搏液实现心脏骤停。在实验组II中，通过间歇性输注冷晶体停搏液实现心脏骤停。在对照组中，心脏进行常温缺氧停搏45分钟。操作完成后，采集左心室侧壁的八个样本，用10%甲醛和2.5%戊二醛固定，用于结构和超微结构分析。
结构和超微结构结果表明，与使用间歇性冷晶体停搏液保护的心脏骤停组和对照组相比，接受持续温热含血停搏液保护的心脏骤停组心脏保存更好，细胞改变不那么明显。
与间歇性冷晶体停搏液相比，持续温热含血停搏液在保存心肌结构和超微结构完整性方面更有效。
